Validated simultaneous high-performance thin-layer chromatography‒mass spectrometry method for analysis of citalopram prochlorperazine, midazolam, and chlorodiazepoxide in urine for forensic analysis

Drug breakthroughs and development have been a great blessing to mankind. Problem arises when these are used in non-medical context such as use for recreational purposes, using drugs outside the label directions, with malicious intent found homicidal cases. Young generations get trapped into vicious cycle of drug addiction that has deleterious effects on their mind body. Drugs including psychomotor drugs, opioids, narcotics, inhalants, stimulants, central nervous system depressants easily accessible. Forensic science plays crucial role investigation pertaining abuse overdose. Complex biological non-biological matrices submitted forensic laboratories analysis interpretation drugs/toxicants/poisons human body environment. The aim this study was develop method determination various prescription urine high-performance thin-layer chromatography‒mass spectrometry (HPTLC‒MS) technique. New combination solvent systems tried simultaneous detection frequently urine. developed validated HPTLC‒MS quantitative estimation benzodiazepines, anti-depressants, tranquilizers common all drugs. Mobile phase cyclohexane‒toluene‒diethylamine (7:1.5:1.5, V/V) one drop strong ammonia solution mobile phase. Method validation carried out according International Council Harmonization guidelines. present being simple, sensitive, precise, accurate can be specimens.